Composite

Part:BBa_K1615055

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)

Linker-CBDcipA-linker fused to Heroin Esterase in RFC25

Heroin esterase (BBa_K1615045) fused to the C terminal of CBDcipA with N and C terminal linkers (BBa_K1615111). If folded properly, this allows heroin esterase to deacetylate heroin while immobilised on cellulose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 738
    Illegal BamHI site found at 1494
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None